An example of quantifying RNA-seq expression with RSEM on Odyssey cluster

A best-practices pipeline for de novo transcriptome assembly with Illumina paired-end reads using Trinity

This tutorial will help users go from raw FASTQ sequencing files to analysis-read VCF files.

Our core sequencing service has two Illumina HiSeqs and a MiSeq. The FAS Informatics group is committed to providing a fast basecalling and QC service for all users of the facility. Beyond this we strongly encourage people to get in contact with us and we’ll be happy to discuss things further. We aim to work alongside researchers with the intention of spreading knowledge and enabling people to run analyses themselves. As we grow we are building workflows and pipelines to enable more people to be self sufficient in bioinformatics analysis.

How to add a new Sequencing Price to the Bauer Core MiniLIMS system

Introduction to sequencing in the Bauer Core Oct2014Workshop_BauerCore.pdf

Library prep for Bauer Core sequencing Oct2014Workshop_LibraryPrep.pdf

This tutorial provides useful tips for setting up sequencing experiments to provide the best results after analysis.

Multiplexing allows several samples to be sequenced in the same lane, but there can be problems with very short barcodes.